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Download the protocol documents, field- and laboratory sheets, and data entry spreadsheets from figshare:

Protocol Link
Predation Assay https://doi.org/10.25573/serc.14717802


‘Squidpops’ provide a simple, quick, standardized assay of fish predation intensity. Fish predation is important in nearly all marine ecosystems as it structures communities and is how production moves up the food web. The goal of this protocol is to establish a simple, standard method to quantify the relative intensity of feeding by generalist predators that is comparable across a wide range of sites and conditions.

To get started, use the links above to download the protocol, datasheet, and data entry template files.

Measured Parameters

Feeding intensity by generalist predators, measured as:

  • Bait loss after 1 hour
  • Bait loss after 24 Hours

Data submission

Use the data entry template to transfer data from the field datasheets into Excel and then submit data to marinegeo-data@si.edu.

Additional Resources

Tutorial Video

Selected literature

Duffy, J. E., S. L. Ziegler, J. E. Campbell, P. M. Bippus, and J. S. Lefcheck. 2015. Squidpops: A Simple Tool to Crowdsource a Global Map of Marine Predation Intensity. PLoS ONE 10:e0142994.

Gauff, R. P. M., S. Bejarano, H. H. Madduppa, B. Subhan, E. M. A. Dugény, Y. A. Perdana, and S. C. A. Ferse. 2018. Influence of predation risk on the sheltering behaviour of the coral-dwelling damselfish, Pomacentrus moluccensis. Environmental Biology of Fishes 101:639-651.

Gross, C., C. Donoghue, C. Pruitt, A. C. Trimble, and J. L. Ruesink. 2017. Taxonomic and functional assessment of mesopredator diversity across an estuarine habitat mosaic. Ecosphere 8:e01792.

Gusmao, J. B., M. R. Lee, I. MacDonald, N. C. Ory, J. Sellanes, Les Watling, and M. Thiel. 2018. No reef-associated gradient in the infaunal communities of Rapa Nui (Easter Island): Are oceanic waves more important than reef predators? Estuarine Coastal and Shelf Science 210:123-131.

Lefcheck, J. S., S. J. Brandl, P. L. Reynolds, A. R. Smyth, and S. T. Meyer. 2016. Extending Rapid Ecosystem Function Assessments to Marine Ecosystems: A Reply to Meyer. Trends in ecology & evolution 31:251–253.

Rodemann, J. R., and S. J. Brandl. 2017. Consumption pressure in coastal marine environments decreases with latitude and in artificial vs. natural habitats. Marine ecology progress series. Oldendorf 574:167–179.